General Cell Based Assays
Echelon Biosciences Research Labs GENERIC PHOSPHATASE ASSAY KIT
Echelons Malachite Green Assay Kit is designed for the in vitro measurement of lipid and protein phosphatase activity. The liberated phosphate forms a colored complex with molybdate/malachite green, which is quantified by reading the absorbance at 620 nm. The assay uses water-soluble lipid substrates so there is no need to make phospholipid vesicles. The assay is fast, convenient and offers a flexible non-radioactive alternative for measuring specific phosphatase activities. The Malachite Green Assay kit can measure as little as 50 pmoles of free phosphate and has a high level of accuracy in the range of 200 to 2,000 pmol of phosphate per assay point. The kit includes enough reagents for 192 samples, standards, and controls. This item is non returnable
Cayman Chemical TrnsglutamInase asy Stop10mL
Transglutaminase Assay Stop Solution has been tested and formulated to work exclusively with Cayman’s Transglutaminase Activity Assay Kit (Item No. 702250).
Eurofins Discoverx cAMP Hunter eXpress LTB4R CHO-K1 GPCR Assay, 10-Plate Kit. (95-0085E2CP2L)
The cAMP Hunter LTB4R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. leukotriene B4) based activation of LTB4R GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type LTB4R, a naturally Gi/Go coupled GPCR. Upon activation of LTB4R, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTHR1 Activated GPCR Internalization Assay, 10pl Kit. (93-0770E3CP0L)
The PathHunter PTHR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTHR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged PTHR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting PTHR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Rat IL-6 ASY
The Meso Scale Discovery R-PLEX Rat IL-6 Assay is a multiplex immunoassay panel that allows simultaneous quantitative measurement of rat interleukin 6 (IL-6) in serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision with low sample volume requirements The assay requires 50 L or less of sample and has a testing time of 3 5 hours The lower limit of detection for rat IL-6 is 1 3 pg/mL This assay is for research use only and is not intended for diagnostic procedures It is available as a singleplex assay or as part of a multiplex panel
LICOR GAR CellTag™ 700 Stain ICW Kit II
GAR CellTag 700 Stain ICW Kits provide detection reagents for cell-based In-Cell Western™ Assays. This cost-effective normalization method makes quantification of the target protein more precise. CellTag 700 Stain accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number. This kit includes reagents to perform an In-Cell Western assay and CellTag 700 Stain to normalize well-to-well variations in cell number. CellTag 700 accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number. Kit Components include IRDye 800CW Goat anti-Rabbit Secondary Antibody (0.5 mg), Intercept (TBS) Blocking Buffer (1 x 500 mL), CellTag 700 Stain (2 x 10 nmol), and Kit Protocol
Eurofins Discoverx PathHunter eXpress P2RY2 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0628E3CP5M)
The PathHunter P2RY2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. UTP) based activation of P2RY2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY2 and EA-tagged β-Arrestin-2. Activation of P2RY2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter™ Mouse TAAR1 Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Mouse TAAR1 Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure mouse TAAR1 (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress EphB2 Functional Assay, 10-Plate Kit. (93-0848E3CP16L)
The PathHunter eXpress EphB2 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphB2 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged EphB2 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical Glycerol CellBased Assay Kit
Cayman’s Glycerol Cell-Based Assay provides a convenient tool for studying triglycerides/fatty acid cycling and its regulation in adipocytes or hepatocytes. This kit will allow investigators to screen compounds involved in lipid storage and metabolism. Chloroquine is included in the kit as a positive control for screening pharmaceuticals that induce lipid droplet accumulation and free glycerol release from hepatocytes.
Eurofins Discoverx PathHunter eXpress GLP1R CHO-K1 β-Arrestin-1 GPCR Assay, 2-Plate Kit. (93-0914E2CP2M)
The PathHunter GLP1R (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP1R and EA-tagged β-Arrestin-1. Activation of GLP1R stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CALCR-RAMP2 (AMY2) CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0267E2CP0L)
The PathHunter CALCR-RAMP2 (AMY2) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. calcitonin) based activation of CALCR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCR and EA-tagged β-Arrestin-2, along with untagged modulating protein RAMP2. Activation of CALCR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences Red hydrogen peroxide assay kit (1Kit)
Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling. Applications: Fluorescence microscopy, HTS .
Eurofins Discoverx PathHunter Human IL-17A (IL17RA/IL17RC) Dimerization Cell-Based Assay Kit (HEK 293)
The PathHunter Human IL-17A (IL17RA/IL17RC) Stable Cell Line Dimerization Assay (HEK 293) measures ligand-induced dimerization of IL17RA/IL17RC. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
TRIBIOSCIENCE INC Cytochrome C Oxidase Activity Assay 100 Tests
The colorimetric assay in this kit is based on the observation of the decrease in absorbance at 550 nm of ferrocytochrome c caused by its oxidation to ferricytochrome c by cytochrome c oxidase. It is suitable for the detection of mitochondrial outer membrane integrity and for the detection of mitochondria in subcellular fractions.